Antioxidant, Anti-Collagenase, Anti-Elastase and Anti-Tyrosinase Activities of an Aqueous Cosmos caudatus Kunth (Asteraceae) Leaf Extract

Zakaria NN. Azwanida¹, Okello E. Jonathan², Howes Melanie-Jaynes³

¹Faculty of Agro‐based Industry, University Malaysia Kelantan, Jeli 17600, Kelantan, Malaysia

²Human Nutrition Research Centre, Institute of Cellular Medicine, The Medical School, Newcastle University, Newcastle upon Tyne NE2 4HH, UK

³Natural Capital and Plant Health, Royal Botanic Gardens, Kew, Richmond TW9 3AB, UK

Corresponding Author: azwanida@umk.edu.my; Tel: +609-9477020 (10807)

Recieved Date: October 19, 2020; Accepted Date: December 21, 2020; Published Date: 02 January 2021

Citation: Azwanida ZNN; Jonathan OE, Melanie-Jaynes H. Antioxidant, Anti-collagenase, Anti-elastase and Anti-tyrosinase Activities of an Aqueous Cosmos caudatus Kunth (Asteraceae) Leaf Extract. Trop J Nat Prod Res. 2020; 4(12): 1124-1130.  https://doi.org/10.26538/tjnpr/v4i12.15

Copyright: © 2020 Azwanida et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

ABSTRACT

Cosmos caudatus Kunth (Asteraceae) or wild cosmos is traditionally used as a culinary and medicinal herb. The leaves and shoots of the plant are reputed to promote a younger skin complexion if consumed regularly. However, research to support its potential use as a cosmeceutical is lacking. This study investigated the antioxidant, anti-collagenase, anti-elastase and anti-tyrosinase activities of C. caudatus leaf water extract (CCW) in vitro to scientifically evaluate its potential as a cosmeceutical. The antioxidant activity was measured using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays, anti-collagenase and anti-elastase activity were measured using fluorescence assays and anti-tyrosinase assay was measured by colorimetric assay. The DPPH and ABTS assays showed CCW to have IC50 values of 163.6 and 57.2 µg/mL, respectively. Significant collagenase, elastase and tyrosinase inhibitions were observed with 51.3, 63.7 and 72.7% inhibition, respectively at 1000 µg/mL CCW treatment, compared with controls. Flavonol glycosides derived from quercetin and kaempferol, and flavone C-glycosides derived from apigenin were the main compounds identified in CCW using high resolution LC-UV-MS/MS. These results suggest the potential application of CCW as a cosmeceutical with antioxidant, anti-collagenase, anti-elastase, anti-tyrosinase activities.