Chemical Composition, Antioxidant Potential and Antimicrobial Properties of the Essential Oils of Haplophyllum tuberculatum (Forsskal) A. Juss from Morocco

Abdelkrim Agour1*, Ibrahim Mssillou1, Hamza Saghrouchni2, Amina Bari2, Badiaa Lyoussi1, Elhoussine Derwich1,3
1Laboratory of Natural Substances, Pharmacology, Environment, Modeling, Health & Quality of Life, Department of Biology, Faculty of Sciences, Sidi Mohamed Ben Abdellah University, Fez, Morocco
2Laboratory of Biotechnology, Environment, Agri-food and Health, Department of Biology, Faculty of Sciences, Sidi Mohamed Ben Abdellah and University, Fez, Morocco
3Unity of GC/MS and GC, City of Innovation, Sidi Mohamed Ben Abdellah University, Fez, Morocco

Corresponding Author: abdelkrimagour1@gmail.com; Tel: +212 777801988
Recieved Date: October 16, 2020; Accepted Date: December 20, 2020; Published Date: 02 January 2021
Citation: Agour A, Mssillou I, Saghrouchni H, Bari A, Lyoussi B and Derwich E. Chemical Composition, Antioxidant Potential and Antimicrobial Properties of the Essential Oils of Haplophyllum tuberculatum (Forsskal) A. Juss from Morocco. Trop J Nat Prod Res. 2020; 4(12): 1108-1115.  https://doi.org/10.26538/tjnpr/v4i12.13
Copyright: © 2020 Agour et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
ABSTRACT

Essential oils are secondary metabolites of plants which have shown very important biological activities. The chemical composition, antioxidant and antimicrobial activity of essential oils isolated from Haplophyllum tuberculatum (Forsskal) A. Juss are the subject of this study. The essential oils was extracted by hydrodistillation, GC/MS and GC-FID were used to determine the chemical composition of the essential oils studied. Two tests were used to assess antioxidant power DPPH (2,2-diphenyl-1-picrilhidrazil) test and TAC (Total antioxidant capacity) assays. Agar diffusion and microdilution were used to examine the antimicrobial activity of these oils. The GC/MS and GC-FID analysis reveal 50 components. The major component was Farnesyl bromide (37.02%) followed by santolina triene (10.26%), terpinene-4-ol (6.13%) and limonene (2.26%). The scavenging capacity of DPPH by essential oils (IC50 = 3.2395 ± 0.009 mg/mL) was lower than that of ascorbic acid (IC50= 2.8195 ± 0.008 ?g/mL), but the measurement of its TAC revealed the presence of a significant amount of antioxidants in the oils studied (617.95 ± 0.589 mg EAA/g extract). S. aureus (bacteria G +) appears the most sensitive (24 mm). The values of the MIC are proportional to the values of the diameters of the inhibition zone, the oil was very active on S. aureus (MIC = 5.91?g/mL). E. coli and P. aeruginosa were the least sensitive (MIC = 94.6 ?g/mL). The results of this study showed that this oil can be an alternative source of antimicrobials to combat strains with a problem of resistance to conventional antibiotics.

Keywords: H. tuberculatum, Essential oils, GC/MS, Antioxidant, Antimicrobial activity.
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