Antibacterial Activity and Stability Evaluation of “Apo-taat” Remedy Extract for Inhibiting Diarrhea-Causing Bacteria

Sumalee Panthong1,4*, Intouch Sakpakdeejaroen1, Pranporn Kuropakornpong2, Jaiboonya Jaicharoensub3, Arunporn Itharat1,4
1Department of Applied Thai Traditional Medicine, Faculty of Medicine, Thammasat University, Pathumthani 12120, Thailand
2Student of Doctor of Philosophy in Medical Science Program, Faculty of Medicine, Thammasat University, Pathumthani 12120, Thailand
3Student of Master of Science Program in Applied Thai Traditional Medicine, Faculty of Medicine, Thammasat University, Pathumthani 12120, Thailand
4Centre of Excellence in Applied Thai Traditional Medicine Research (CEATMR), Thammasat University, Pathumthani 12120, Thailand

Corresponding Author: psumalee@tu.ac.th; Tel: 6629269734
Recieved Date: October 15, 2020; Accepted Date: December 18, 2020; Published Date: 02 January 2021
Citation: Panthong S, Sakpakdeejaroen I, Kuropakornpong P, Jaicharoensub J, Itharat A. Antibacterial Activity and Stability Evaluation of “Apo-taat” Remedy Extract for Inhibiting Diarrhea-Causing Bacteria. Trop J Nat Prod Res. 2020; 4(12): 1101-1107.  https://doi.org/10.26538/tjnpr/v4i12.12
Copyright: © 2020 Panthong et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
ABSTRACT

Apo-taat, a plant-based, Thai traditional medicine that contains Caesalpinia sappan and Phyllanthus emblica, is used for treating diarrhea. In the present study, the ingredients of the Apo-taat remedy were extracted, analysed and investigated for antibacterial activity and stability testing under forced degradation and accelerated conditions. Apo-taat extract showed good antibacterial activity against Escherichia coli, Pseudomonas aeruginosa, Salmonella enterica subsp. enterica serovar Typhi, Shigella dysenteriae, Staphylococcus aureus, methicillin-resistant S. aureus and Bacillus subtilis with MIC values of 0.313 to 1.25 mg/mL. The main chemical constituent of Apo-taat extract was brazilin (51.94 mg/g of dried extract) and gallic acid (7.30 mg/g of dried extract). Brazilin and gallic acid were unstable under acid and alkaline conditions but stable under oxidation and thermal degradation and over six months. These results can be concluded that Apo-taat showed good antibacterial activity and stable under oxidation condition, thermal condition and long-term storage at room temperature.

Keywords: Apo-taat remedy, Anti-bacterial activity, Caesalpinia sappan, Phyllanthus emblica, Stability testing.
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